🔬 pH Buffer Calculator

Professional buffer solution calculator for laboratory and research use. Calculate precise buffer solutions based on the Henderson-Hasselbalch equation.

Buffer Calculation Tool

Choose Calculation Mode

Select how you want to prepare your buffer solution

Buffer Parameters

Buffer SystemChoose a pre-defined buffer system or select 'Custom' to enter your own pKa

Desired pHTarget pH for your buffer (typically 6.0-8.0 for biological work)

Final Volume (L)Total volume of buffer to prepare in liters (e.g., 0.1 L = 100 mL)

Total Buffer Concentration (M)Final molarity of the buffer. Common values: 0.01-0.1 M for cell culture, 0.1-1.0 M for biochemistry

Mode-Specific Parameters

Stock Solution Concentrations

Specify the molarity of your stock solutions

Acid Stock Concentration (M)Molarity of your acid stock solution (e.g., 1.0 M = 1 mole per liter)

Base Stock Concentration (M)Molarity of your base stock solution (e.g., 1.0 M = 1 mole per liter)

Calculation Results

Enter your buffer parameters to see detailed calculations

About Buffer Calculations

Henderson-Hasselbalch Equation

Our calculator uses the Henderson-Hasselbalch equation to determine the precise ratio of acid to conjugate base needed for your desired pH. This fundamental equation is essential for buffer preparation in biochemistry and molecular biology.

Common Buffer Systems

• Tris Buffer: pH 7.0-9.0, ideal for protein work
• PBS Buffer: pH 7.4, cell culture standard
• Phosphate Buffer: pH 6.0-8.0, versatile system
• HEPES Buffer: pH 6.8-8.2, biological buffering